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stemdifftm hematopoietic kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc stemdifftm hematopoietic kit
    Stemdifftm Hematopoietic Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemdifftm+hematopoietic+kit/stemdifftm+hematopoietic+kit/pmc12276663-93-15-18
    Average 90 stars, based on 1 article reviews
    stemdifftm hematopoietic kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transduction:

    Article Title: Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles.
    Article Snippet: .. The next day, they are transduced for 48 hourswith 2doses of PE-VLPs at 24-hour intervals in 200 μLof hiPSC mediumwithout Y-27632 for 4 hours, afterwhich 500 μL of freshhiPSC medium is added. hiPSC-derived HSCs and transduction with PE-VLPs AG08C5 iPSCs (#PGNMi001-A on hPSCreg site) were differentiated for 9 or 12 days using the STEMdiffTM Hematopoietic Kit (#05310, STEMCELL Technologies, Inc.). ..

    other:

    Article Title: A glia-enriched stem cell 3D model of the human brain mimics the glial-immune neurodegenerative phenotypes of multiple sclerosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BSA Molecular Biology Grade New England Biolabs Cat# B2000S XbaI restriction enzyme New England Biolabs Cat# #R0145 NheI restriction enzyme New England Biolabs Cat# R3131 MluI restriction enzyme New England Biolabs Cat# R3198 SpeI restriction enzyme New England Biolabs Cat# R3133 T4 DNA Polymerase Promega Cat# M4211 Antarctic Phosphatase New England Biolabs Cat# M0289S T4 DNA-ligase New England Biolabs Cat# M0202S SOC medium Invitrogen Cat# 15544034 Ampicillin Roche Cat# 10835269001 Triiodo-L-thyronine (T3) Sigma Aldrich Cat# T6397 Recombinant human PDGF Preprotech Cat# 100-13A Recombinant human NT3 Preprotech Cat# 450-03 Recombinant human GDNF Sino Biological Inc Cat# 10561-HNCH Recombinant human IGF-1 Preprotech Cat# 100-11 Recombinant human BDNF Sino Biological Inc Cat# 50240-MANS dcAMP analog Sigma Aldrich Cat# D0627 Doxycycline hydrochloride Sigma Aldrich Cat# D3447 Triton X-100 reagent Sigma Aldrich Cat# 93420 DAPI fluorescence reagent for DNA Thermo Fisher Scientific Cat# D1306 RapiClearTM 1.47 SUNJin Lab Cat# RC147001 Dako fluorescent mounting medium Agilent Cat# S3023 Epon resin Electron Microscopy Science Cat# 50-980-342 Papain STEMCELL Cat# 074ss66 DNAase I for cell culture STEMCELL Cat# 07900 EcoRI New England Biolabs Cat# R3101 AflIII restriction enzyme New England Biolabs Cat# R0541S NotI restriction enzyme New England Biolabs Cat# R0189S EcoRV restriction enzyme New England Biolabs Cat# R0195S Iscove’s modified Dulbecco medium Sigma Aldrich Cat# I3390 Fetal Bovine Serum Euroclone Cat# ECS0180L Ovomucoid protease inhibitor Worthington Biochemical Cat#LK003182 Critical commercial assays Sendai virus kit Cytotune Invitrogen Cat# A16517 Plasmid DNA Extraction mini kit Cat# DE-034 Xtra Maxi EF Macherey-Nagel Cat# 740424.50 STEMdiffTM Hematopoietic Kit STEMCELL Cat# 05310 STEMdiffTM Micorglia Differentiation Kit STEMCELL Cat# 100-0019 STEMdiffTM Microglia Maturation Kit STEMCELL Cat# 100-0020 RNeasy Mini kit Qiagen Cat# 74106 Thermo Script RT-PCR Invitrogen Cat# 11146-024 SuperScript IV First-Strand Synthesis System Thermo Fisher Scientific Cat# 18091050 Deposited data scRNA-data of this study Deposited to GEO GSE233295 scRNA-seq for human adult cortex Hao et al., 2021.18 GSE164378 scRNA-seq for human fetal brain Cao et al., 2020.19 GSE156793 scRNA-seq for human cortical organoids Birey et al., 2017.20 SRA: SRP096997 scRNA-seq for human cortical organoids Fiddes et al., 2018.21 SRA: SRP121791 (Continued on next page) e2 Cell Reports Medicine 5, 101680, August 20, 2024

    Article Title: Sex differences on laser-induced choroidal neovascularization and short-chain fatty acid treatment in a mouse model
    Article Snippet: Firstly, hiPSC clusters were differentiated into hematopoietic progenitor cells (HPCs) in Geltrex-coated plates using the STEMdiffTM hematopoietic kit (STEMCELL Technologies).

    Modification:

    Article Title: BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesis
    Article Snippet: .. For erythroid differentiation from ESCs (embryonic stem cells), CD34 + HSPCs were induced by STEMdiffTM Hematopoietic Kit (Stem Cell Technologies, 05310) with minor modification. ..

    Marker:

    Article Title: Microglial APOE3 Christchurch protects neurons from Tau pathology in a human iPSC-based model of Alzheimer's disease.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Iba1 antibody Abcam Cat # ab5076; RRID: AB_2224402 Anti-TREM2 antibody [EPR20243] Abcam Cat # ab209814; RRID: AB_3095849 MAP2 antibody - Neuronal Marker Abcam Cat # ab5392; RRID: AB_2138153 Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Thermo Fisher Scientific Cat # MN1020; RRID: AB_223647 Polyclonal Rabbit anti-TUJ1 Covance Cat # PRB-435P; RRID: AB_291637 Anti-Tau (T22), oligomeric Antibody Sigma Aldrich Cat # ABN454; RRID: AB_2888681 Anti-TFRC Antibody Santa Cruz Biotechnology Cat # SC-32272; RRID: AB_627167 Anti-Apolipoprotein E Goat pAb Millipore Cat # 178479; RRID: AB_10682965 Chemicals, peptides, and recombinant proteins ROCK inhibitor Y-27632 dihydrochloride Reprocell Cat # 04-0012-10 Retinoic acid Sigma Aldrich Cat # R2625 CHIR99021 Cellagen Technology Cat # C2447-2s LDN-193189 Cellagen Technology Cat # C5361-2s SB431542 Cellagen Technology Cat # C7243-5 LipidSpotTM Lipid Droplet Stains Biotium Cat # 70065 BODIPYTM 581/591 C11 (Lipid Peroxidation Sensor) Thermo Fisher Scientific Cat # D3861 Matrigel Corning Cat # 354230 N2 supplement Life Technologies Cat # 17502048 B27 supplement Thermo Fisher Scientific Cat # 17504044 Essential 8 Medium Life Technologies Cat # A1517001 Human Recombinant Insulin solution Sigma Aldrich Cat # I9278 MEM NEAA Thermo Fisher Scientific Cat # 11140076 DMEM-F12 Gibco Cat # 11330-032 BrainPhys medium STEMCELL Technologies Cat # 05790 Liproxstatin-1 hydrochloride Tocris Bioscience Cat # 6113 Ferrostatin-1 MedChemExpress Cat # HY-100579 Erastin MedChemExpress Cat # HY-15763 GDNF PeproTech Cat # 450-10 BDNF PeproTech Cat # 450-02 Apolipoprotein E/APOE Protein, Human, Recombinant Sino biological Cat # 10817-H30E Dibutyryl-cAMP Sigma Aldrich Cat # D0627 STEMdiffTM Hematopoietic Kit STEMCELL Technologies Cat # 05310 Recombinant Human GM-CSF (carrier-free) Biolegend Cat # 572904 Transforming Growth Factor b 1 Human Recombinant TGFB1 Prospec Cat # CYT-716 Recombinant Human IL-34 PeproTech Cat # 200-34 ATRA Sigma Aldrich Cat # R2625 (Continued on next page) 16 Cell Reports 43, 114982, December 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER EGF PeproTech Cat # 100-15 FGF PeproTech Cat # 100-18B Trizol Invitrogen Cat # 15596018 Gibco GlutaMAX Supplement Invitrogen Cat # 35050079 DyNAmo Flash SYBR Green qPCR mix Thermo Fisher Scientific Cat # F416 PMSF Roche Cat # 837091 pHrodoTM Red E. coli BioParticlesTM Conjugate for Phagocytosis Invitrogen Cat # P35361 pHrodoTM Red, succinimidyl ester (pHrodoTM Red, SE) Thermo Fisher Scientific Cat # P36600 Critical commercial assays Tetro cDNA synthesis kit Bioline Cat # Bio-65043 Amaxa P3 primary 4D-Nucleofector X kit L Lonza Cat # V4XP-3024 Stem Cell Technologies STEMdiffTM Hematopoietic Kit STEMCELL Technologies Cat # 05310 Deposited data RNA-seq data of microglia This study GSE240609 Experimental models: Cell lines Human: AG14048 Coriell Institute Cat # AG14048 Human: AG14048 APOECh This study N/A Human: ADRC8 UC Irvine N/A Human: ADRC8 APOECh This study N/A Human: ADRC8 TFRC knock-in This study N/A Human: ADRC8 APOECh TFRC knock-in This study N/A u ApoE3 Harvard N/A a ApoECh Harvard N/A APOE R136S SNV/SNV "CHRISTCHURCH" (A) Jackson lab Cat # JIPSC1264 KOLF2.1J Jackson lab Cat # JIPSC1000 Oligonucleotides PSEN1 sgRNA 50- TAT GCT GGT TGA AAC AGC TC-30 IDT, Coralville N/A PSEN1 ssODN 50-GAT TTA GTG GCT GTT TTG TGT CCG AA A GGT CCA CTT CGT ATG CTG GTT GAG ACC GCT CAG GAG AGA AAT GCA ACG CTT TTT CCA GCT CTC ATT TAC TCC T-30 IDT, Coralville N/A APOECh sgRNA 50- CCA GAG CAC CGA GGA GCT GC-30 IDT, Coralville N/A APOECh ssODN 50-GCG GCG AGG TGC AGG CCA TGC TCG GCC AGA GCA CCG AGG AGC TGC GCG TGA GCC TCG CCT CCC ACC TGC GCA AGC TGC GTA AGC GGC TCC TCC GCG ATG C-30 IDT, Coralville N/A TMEM119-F: 50- GGA TAG TGG ACT TCT TCC GCC A-30 IDT, Coralville N/A TMEM119-R: 50- GGA AGG ACG ATG GGT AAT AGG C -30 IDT, Coralville N/A P2RY12-F: 50- TGC CAA ACT GGG AAC AGG ACC A -30 IDT, Coralville N/A P2RY12-R: 50- TGG TGG TCT TCT GGT AGC GAT C -30 IDT, Coralville N/A IL1b-F: 50-GCA GGC CGC GTC AGT TGT TG-30 IDT, Coralville N/A IL1b-R: 50-CCC GGA GCG TGC AGT TCA GT-30 IDT, Coralville N/A ATG7-F: 50- CGT TGC CCA CAG CAT CAT CTT C -30 IDT, Coralville N/A ATG7-R:50 CAC TGA GGT TCA CCA TCC TTG G -30 IDT, Coralville N/A SAT1-F: 50- CTT TGG AGC CAC CTC TCT ACA G -30 IDT, Coralville N/A (Continued on next page) Cell Reports 43, 114982, December 24, 2024 17

    Recombinant:

    Article Title: Microglial APOE3 Christchurch protects neurons from Tau pathology in a human iPSC-based model of Alzheimer's disease.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Iba1 antibody Abcam Cat # ab5076; RRID: AB_2224402 Anti-TREM2 antibody [EPR20243] Abcam Cat # ab209814; RRID: AB_3095849 MAP2 antibody - Neuronal Marker Abcam Cat # ab5392; RRID: AB_2138153 Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8) Thermo Fisher Scientific Cat # MN1020; RRID: AB_223647 Polyclonal Rabbit anti-TUJ1 Covance Cat # PRB-435P; RRID: AB_291637 Anti-Tau (T22), oligomeric Antibody Sigma Aldrich Cat # ABN454; RRID: AB_2888681 Anti-TFRC Antibody Santa Cruz Biotechnology Cat # SC-32272; RRID: AB_627167 Anti-Apolipoprotein E Goat pAb Millipore Cat # 178479; RRID: AB_10682965 Chemicals, peptides, and recombinant proteins ROCK inhibitor Y-27632 dihydrochloride Reprocell Cat # 04-0012-10 Retinoic acid Sigma Aldrich Cat # R2625 CHIR99021 Cellagen Technology Cat # C2447-2s LDN-193189 Cellagen Technology Cat # C5361-2s SB431542 Cellagen Technology Cat # C7243-5 LipidSpotTM Lipid Droplet Stains Biotium Cat # 70065 BODIPYTM 581/591 C11 (Lipid Peroxidation Sensor) Thermo Fisher Scientific Cat # D3861 Matrigel Corning Cat # 354230 N2 supplement Life Technologies Cat # 17502048 B27 supplement Thermo Fisher Scientific Cat # 17504044 Essential 8 Medium Life Technologies Cat # A1517001 Human Recombinant Insulin solution Sigma Aldrich Cat # I9278 MEM NEAA Thermo Fisher Scientific Cat # 11140076 DMEM-F12 Gibco Cat # 11330-032 BrainPhys medium STEMCELL Technologies Cat # 05790 Liproxstatin-1 hydrochloride Tocris Bioscience Cat # 6113 Ferrostatin-1 MedChemExpress Cat # HY-100579 Erastin MedChemExpress Cat # HY-15763 GDNF PeproTech Cat # 450-10 BDNF PeproTech Cat # 450-02 Apolipoprotein E/APOE Protein, Human, Recombinant Sino biological Cat # 10817-H30E Dibutyryl-cAMP Sigma Aldrich Cat # D0627 STEMdiffTM Hematopoietic Kit STEMCELL Technologies Cat # 05310 Recombinant Human GM-CSF (carrier-free) Biolegend Cat # 572904 Transforming Growth Factor b 1 Human Recombinant TGFB1 Prospec Cat # CYT-716 Recombinant Human IL-34 PeproTech Cat # 200-34 ATRA Sigma Aldrich Cat # R2625 (Continued on next page) 16 Cell Reports 43, 114982, December 24, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER EGF PeproTech Cat # 100-15 FGF PeproTech Cat # 100-18B Trizol Invitrogen Cat # 15596018 Gibco GlutaMAX Supplement Invitrogen Cat # 35050079 DyNAmo Flash SYBR Green qPCR mix Thermo Fisher Scientific Cat # F416 PMSF Roche Cat # 837091 pHrodoTM Red E. coli BioParticlesTM Conjugate for Phagocytosis Invitrogen Cat # P35361 pHrodoTM Red, succinimidyl ester (pHrodoTM Red, SE) Thermo Fisher Scientific Cat # P36600 Critical commercial assays Tetro cDNA synthesis kit Bioline Cat # Bio-65043 Amaxa P3 primary 4D-Nucleofector X kit L Lonza Cat # V4XP-3024 Stem Cell Technologies STEMdiffTM Hematopoietic Kit STEMCELL Technologies Cat # 05310 Deposited data RNA-seq data of microglia This study GSE240609 Experimental models: Cell lines Human: AG14048 Coriell Institute Cat # AG14048 Human: AG14048 APOECh This study N/A Human: ADRC8 UC Irvine N/A Human: ADRC8 APOECh This study N/A Human: ADRC8 TFRC knock-in This study N/A Human: ADRC8 APOECh TFRC knock-in This study N/A u ApoE3 Harvard N/A a ApoECh Harvard N/A APOE R136S SNV/SNV "CHRISTCHURCH" (A) Jackson lab Cat # JIPSC1264 KOLF2.1J Jackson lab Cat # JIPSC1000 Oligonucleotides PSEN1 sgRNA 50- TAT GCT GGT TGA AAC AGC TC-30 IDT, Coralville N/A PSEN1 ssODN 50-GAT TTA GTG GCT GTT TTG TGT CCG AA A GGT CCA CTT CGT ATG CTG GTT GAG ACC GCT CAG GAG AGA AAT GCA ACG CTT TTT CCA GCT CTC ATT TAC TCC T-30 IDT, Coralville N/A APOECh sgRNA 50- CCA GAG CAC CGA GGA GCT GC-30 IDT, Coralville N/A APOECh ssODN 50-GCG GCG AGG TGC AGG CCA TGC TCG GCC AGA GCA CCG AGG AGC TGC GCG TGA GCC TCG CCT CCC ACC TGC GCA AGC TGC GTA AGC GGC TCC TCC GCG ATG C-30 IDT, Coralville N/A TMEM119-F: 50- GGA TAG TGG ACT TCT TCC GCC A-30 IDT, Coralville N/A TMEM119-R: 50- GGA AGG ACG ATG GGT AAT AGG C -30 IDT, Coralville N/A P2RY12-F: 50- TGC CAA ACT GGG AAC AGG ACC A -30 IDT, Coralville N/A P2RY12-R: 50- TGG TGG TCT TCT GGT AGC GAT C -30 IDT, Coralville N/A IL1b-F: 50-GCA GGC CGC GTC AGT TGT TG-30 IDT, Coralville N/A IL1b-R: 50-CCC GGA GCG TGC AGT TCA GT-30 IDT, Coralville N/A ATG7-F: 50- CGT TGC CCA CAG CAT CAT CTT C -30 IDT, Coralville N/A ATG7-R:50 CAC TGA GGT TCA CCA TCC TTG G -30 IDT, Coralville N/A SAT1-F: 50- CTT TGG AGC CAC CTC TCT ACA G -30 IDT, Coralville N/A (Continued on next page) Cell Reports 43, 114982, December 24, 2024 17



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